Centre Européen
de Recherche et d'Enseignement
des Géosciences de l'Environnement

PLATFORM FOR STABLE ISOTOPE ANALYSIS (PANISS)

Laboratory manager : C. Sonzogni
Tel : 04 42 97 17 54/15 31 e-mail : sonzogni@cerege.fr

Scientific supervisor : A. Alexandre
Tel : 04 42 97 17 54/15 42 e-mail : alexandre@cerege.fr

Silicate manager : M. Couapel
Tel: 04 42 97 17 54/ 17 64 email: couapel@cerege.fr

Presentation

CEREGE's stable isotope analysis platform (PANISS) is divided into 4 analytical applications: 1) analyses δ18O and δ13C of carbonates; 2) analyses δ18O and δ17O of silicates and oxides; 3) analyses δ18O, δ17O and δD of water and water vapour; and 4) analyses δ15N, δ13C and δD of organic compounds. 

The data acquired mainly feed CEREGE's research on paleoclimates, water and carbon cycles, and planetology. PANISS also responds to requests for collaborative analyses or services from French and foreign academic organizations. The platform is developing an important training activity.

The platform is under the technical and scientific responsibility of Corinne Sonzogni (CNRS engineer) and Anne Alexandre (CNRS researcher), respectively. A second engineer, Martine Couapel (IRD engineer) is more particularly involved in silicate and water applications.

Laboratory applications

δ18O and δ13C analysis of carbonates

Scientific Objectives

  • Characterization of climate change at high temporal resolution, mainly in tropical to sub-tropical areas (hydrological cycles, ocean conditions)
  • Reconstruction of climate modes past variability  (ENSO type)
  • Evolution of the carbonate system in the ocean in relation to atmospheric CO2 concentrations

Equipment

  • Dual-Inlet Isotope Ratio Mass Spectrometer (DI-IRMS Delta V Plus,Thermo Scientific) coupled to an automated carbonate preparation line (Carbonates Device Kiel IV, Thermo Scientific): δ13C and δ18O on micro-samples from 20-150 μg.
  • Dual-Inlet Isotope Ratio Mass Spectrometer (DI-IRMS Delta Plus Advantage, Thermo Scientific) coupled to an automated carbonate preparation line (Carbonates Device Kiel III): δ13C and δ18O on samples from 60-300 μg.
  • IRIS laser spectrometer (Delta Ray) with URI Connect (Thermo Scientific): δ13C and δ18O on samples ≥ 500 μg and continuous CO2, δ13C on Dissolved Inorganic Carbon in water.

δ18O and δ17O in silicates and oxides

Scientific Objectives

  • Development of new paleoclimatic tracers (e.g. relative atmospheric humidity).
  • Reconstruction of the past dynamics of the continental water cycle: origin and amount of precipitation, lake water balance, evapo-transpiration fluxes at the soil-plant-plant-atmosphere interface.
  • Characterization of weathering and soil processes.
  • Characterization of meteorite and micro-meteorite parent bodies and determination of extraterrestrial matter fluxes.

Equipment

  • Dual-Inlet Isotope Ratio Mass Spectrometer (DI-IRMS Delta V Plus, Thermo Scientific).
  • Manual line for oxygen extraction from silicates, with BrF5 and Infrared CO2 laser (CO2-laser 30W, Merchanteck): δ18O and δ17O on samples of 0.3 - 1.6 mg.
  • Dehydration line under nitrogen flow : dehydration and dehydroxylation of hydrated amorphous silica samples.

δ18O and δD composition of water and water vapor

Scientific Objectives

  • Quantification of fractions at the subsurface-surface interface (including vegetation)-atmosphere.
  • Water cycle tracing at this same interface.

Equipment

  • DI-IRMS  (Delta Plus, Thermo Scientific) coupled to an automated water sample equilibration line (HD Device, Thermo Scientific): measurements δD and δ18O on 3- 5 ml samples.
  • CRDS laser spectrometer (Cavity Ring-Down Spectroscopy, Picarro L1102-i): measurements δD and δ18O on water samples (2 ml) and water vapor.
  • CRDS laser spectrometer (Cavity Ring-Down Spectroscopy, Picarro L2140-i): measurements δD, δ17O and δ18O on 2 ml water samples and water vapour.

δ15N, δ13C and δD of organic compounds

Scientific Objectives

  • Measurement of soil organic carbon turnover for  carbon cycle models
  • Characterization of organic matter stabilization processes in soils
  • Characterization of the impact of climate change on biodiversity and forest litter biodegradation
  • Tracing vegetation changes (δ13C on n-alkanes)
  • Tracing changes in the carbon cycle (δ13C on alkenones)
  • Reconstruction of changes in the hydrological cycle in the continental domain (δD on n-alkanes, triterpenes, etc.).
  • Reconstruction of changes in the hydrological cycle in the marine domain (δDsur n-alkenones, sterols, etc.).

Equipment

  • CF-IRMS Continuous Flow Mass Spectrometer (Delta Plus, Thermo Scientific) connected to a ConFlo III interface to the following equipment:
  • Elemental analyzer (Flash EA, Thermo Scientific) equipped with an automatic sample changer: C, N, δ13C and δ15N measurements on total powders.
  • CF-IRMS continuous flow mass spectrometer (Delta V Plus, Thermo Scientific), connected to a ConFlo IV interface to the following equipment:
  • Elemental analyzer (Flash EA IsoLink CN, SmartEA option, Thermo Scientific) with autosampler: C, N, δ13C and δ15N measurements on total powders.
  • Gas chromatograph (GC 1310-Isolink II, Thermo Scientific) equipped with an automatic sample changer (Triplus RSH, Thermo Scientific), FID detector: measurements δD, δ13C, δ15N on organic molecules separated by gas chromatography.

Operation of the platform

  • For each application a reference team is composed of (i) a reference researcher (list below), (ii) Corinne Sonzogni, engineer in charge of the technical aspects of the platform, and (iii) Martine Couapel, engineer for silicates and water applications.
  • Requests for analyses are made within the framework of a collaboration with a reference researcher or within the framework of a service provision, as far as human and analytical means are available.
  • Users provide samples ready for analysis (contact the referents for details) and undertake to pay the cost of analyses on the basis of the rates provided.

Contacts 

Silicates 

Waters

Carbonates

Organic Compounds